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Projects & Research
Protein Complex Analysis Project Mark Biggin, Principal Investigator (Life Sciences Division) Project Personnel List This project aims to characterize microbes under stress response to conditions commonly found in U.S. Department of Energy (DOE) metal and radionuclide contaminated sites, with an emphasis on high-throughput analysis of microbial multi-protein complexes. The project integrates microbiology (production of tagged protein expression strains and biomass production), multi-protein complex isolation and identification by mass spectrometry, imaging multi-protein complexes by electron microscopy, and computational analysis and modeling that seeks to understand how these complexes control a microorganism’s ability to survive in relevant contaminated environments while reducing metals and radionuclides. Data production and analysis methods will be automated to establish a pipeline that can analyze the majority of stable multi-protein complexes in a microbe as well as a number of unstable complexes. The project, awarded in October 2005, will build on the research and infrastructure of an on-going Genomics:GTL project “Rapid deduction of stress response pathways in metal and radionuclide bacteria” that established the Virtual Institute for Microbial Stress and Survival (VIMSS). |
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