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Projects & Research
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MAGGIE (visit website)
Molecular Assemblies, Genes,
and Genomics Integrated Efficiently
John Tainer, Principal
Investigator (Life Sciences Division)
MAGGIE will provide robust GTL technologies and comprehensive characterizations
to efficiently couple gene sequences and genomic analyses with protein interactions
and thereby elucidate functional relationships and pathways. The operational
principle guiding MAGGIE objectives can be succinctly stated: protein functional
relationships involve interaction mosaics that self-assemble from independent
protein pieces that are tuned by modifications and metabolites. MAGGIE builds
strong synergies among the Components to address long term and immediate GTL
objectives by combining the advantages of specific microbial systems with those
of advanced technologies. The objective for the proposed 5-year MAGGIE Program
is therefore to comprehensively characterize the Protein Complexes (PCs) and
Modified Proteins (MPs) underlying microbial cell biology. A compelling overall
goal is to help reduce the immense complexity of protein interactions to interpretable
patterns though an interplay among experimental efforts of MAGGIE Program members
in molecular biology, biochemistry, biophysics, mathematics, computational science,
and informatics. MAGGIE will address immediate GTL missions by accomplishing
three specific goals: 1) provide a comprehensive, hierarchical map of prototypical
microbial PCs and MPs by combining native biomass and tagged protein characterizations
from hyperthermophiles (temperature-trapping otherwise reversible protein interactions)
with comprehensive systems biology characterizations of a non-thermophilic model
organism, 2) develop and apply advanced mass spectroscopy and SAXS technologies
for high throughput characterizations of PCs and MPs, and 3) create and test
powerful computational descriptions for protein functional interactions. In concert,
MAGGIE investigators will characterize microbial metabolic modularity and provide
the informed basis to design functional islands suitable to transform microbes
for specific DOE missions. |
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