Cloning of Identified Pathways

Diversa Corporation will apply a combination of advanced technologies, including large DNA insert cloning, biopanning, and ultra-high-throughput (UHTP) screening, to study the distribution of stress response pathways in the environment. Diversa routinely constructs large insert fosmid libraries from environmental samples collected world-wide. We will construct large insert fosmid libraries from samples obtained from our collaborators. These will be screened using biopanning, Diversa’s patented technology for enriching desired sequences from DNA libraries by solution-phase hybridization. In this instance, biopanning will involve Gel MicroDroplet (GMD) in situ hybridiza-tion followed by UHTP screening with FACS (Fluorescence Activated Cell Sorting). This approach will be used to capture E. coli fosmid clones carrying genes encoding enzymes responsible for stress response.